Buchi Fraction Collector C-660 Instrukcja Użytkownika Pobierz pdf (Strona 27)

6 Operation

27 C-660 Operation Manual, Version F

Table 6-9: Parameters for conﬁgurationm (cont.)

Parameter Description

Sensitivity The adjustment of various switching sensitivities. Range 4 shows the most sensitive,

Range 1 the most sluggish switching behavior.

This function enables adjustment of the collection function to the relevant slope of the

peak. Peaks with short elution times have steep ﬂanks and the switching decision must

be correspondingly quick. Conversely, peaks with longer elution times have considerably

ﬂatter ﬂanks and are usually somewhat more agitated; such peaks require a more slug-

gish switching decision. The following serves as a rule of thumb:

- Use Range 3 for separations on small columns with diameters of 12...15, range 2 for

larger columns.

- Switch over to the next lower range if the peak detector switches too often. Switch

over to the next highest range if it switches too sluggishly or ‘overlooks’ unambiguous

switching criteria.

Min .

0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16

time (min)

signal (1000 mV =100%)

min

ma x

threshold

min

10 %

min

Fig. 6.7: Example diagram of a detection after peak minimum (min) and peak maximum (max) with a threshold of 10%.

1 2 ... 22 23 24 25 26 27 28 29 30 31 32 ... 35 36

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Buchi Fraction Collector C-660 Instrukcja Użytkownika Strona 27